Linked Life Data

20609425

Source:http://linkedlifedata.com/resource/pubmed/id/20609425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-8-2
pubmed:abstractText
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein) complex, a four-helical bundle composed of syntaxin1 and synaptosome-associated protein 25 (SNAP25) on the plasma membrane and synaptobrevin/VAMP2 (vesicle-associated membrane protein 2) on the vesicle membrane, plays a key role in synaptic exocytosis and facilitates neurotransmission. Disturbances of SNARE proteins were uncovered in some neurodegenerative diseases, neuroendocrine disturbances and even after environmental interventions. In the present study, we evaluated the effects of formaldehyde (FA) inhalation (13.5+/-1.5 ppm, twice 30-min each day for two rounds of 14 consecutive days) on learning and memory in Morris water maze and thereafter explored the SNARE protein levels in hippocampal synaptosomes. The formaldehyde-treated rats showed learning and memory impairment in escape latency and probe trials, without mobility disturbances in Morris water maze. Using western blotting assays, we detected the SNARE proteins in hippocampal synaptosomes and identified decrease of both SNAP25 and VAMP2 after formaldehyde treatment without significant changes of another SNARE protein, syntaxin 1, and synaptic vesicle marker, synaptophysin. Furthermore, the neuronal morphology and number detected in Nissl stain and western blotting assay of neurofilament-150 and synaptophysin were not affected after FA treatment. These results suggested that the specific decrease of SNAP25 and VAMP2 in hippocampal synaptosomes served as a potential contributing mechanism underlying learning and memory impairments after repetitive formaldehyde inhalation treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1873-7544
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1248-54
pubmed:meshHeading
pubmed-meshheading:20609425-Administration, Inhalation, pubmed-meshheading:20609425-Animals, pubmed-meshheading:20609425-Cell Count, pubmed-meshheading:20609425-Cognition Disorders, pubmed-meshheading:20609425-Formaldehyde, pubmed-meshheading:20609425-Hippocampus, pubmed-meshheading:20609425-Learning, pubmed-meshheading:20609425-Male, pubmed-meshheading:20609425-Maze Learning, pubmed-meshheading:20609425-Memory, pubmed-meshheading:20609425-Neurons, pubmed-meshheading:20609425-Rats, pubmed-meshheading:20609425-Rats, Sprague-Dawley, pubmed-meshheading:20609425-Synaptophysin, pubmed-meshheading:20609425-Synaptosomal-Associated Protein 25, pubmed-meshheading:20609425-Synaptosomes, pubmed-meshheading:20609425-Syntaxin 1, pubmed-meshheading:20609425-Time Factors, pubmed-meshheading:20609425-Vesicle-Associated Membrane Protein 2
pubmed:year
2010
pubmed:articleTitle
Disturbances of soluble N-ethylmaleimide-sensitive factor attachment proteins in hippocampal synaptosomes contribute to cognitive impairment after repetitive formaldehyde inhalation in male rats.
pubmed:affiliation
Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education, Ministry of Public Health, Peking University Health Science Center, Beijing 100191, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't