Source:http://linkedlifedata.com/resource/pubmed/id/20592798
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-7-1
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| pubmed:abstractText |
The skin is the only organ that has the capacity to photo-synthesize the biological active vitamin D metabolite 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] from 7-dehydocholesterol (7-DHC), following exposure to ultraviolet (UV)-B irradiation. The aim of the present work was to investigate the capacity of 1,25(OH)(2)D(3) to protect human keratinocytes (HaCaT) and squamous cell carcinoma cell lines (SCL-1) against the hazardous effects of UV-B irradiation. Human keratinocytes (HaCaT) and squamous cell carcinoma cell lines (SCL-1) were pretreated with 1,25(OH)(2)D(3) over 48 hours and then irradiated once with UVB-radiation. We evaluated the results of several assays (colony-forming-unit-culture assay, WST-1-assay and crystal violet assay), comparing viability/proliferation in 1,25(OH)(2)D(3)-pretreated cells with controls that were pretreated with the carrier substance ethanol alone. Additionally, we analyzed the effects of 1,25(OH)(2)D(3) on UV-induced DNA damage in HaCaT-keratinocytes by detection of cyclobutane pyrimidine dimers (CPDs) via dot blot analysis. We prove that 1,25(OH)(2)D(3), in a concentration of 10(-7) M, protects human keratinocytes (HaCaT) as well as squamous cell carcinoma cell lines (SCL-1) against the hazardous effects of UV-B-radiation (100 J/cm(2)-1,000 J/cm(2)) in vitro. Moreover, we demonstrate that the number of CPDs induced in HaCaT-keratinocytes after irradiation with UV-B (100 J/cm(2)-1,000 J/cm(2)) was decreased after pretreatment with 1,25(OH)(2)D(3), as compared to carrier-treated controls. Analysis of the time course revealed that the elimination of UV-B-induced DNA-damage in HaCaT-keratinocytes occurs quicker when cells are pretreated with 1,25(OH)(2)D(3) (as compared to controls). To put it in a nutshell, our data support the hypothesis that 1,25(OH)(2)D(3) protects cultured human keratinocytes against the hazardous effects of UV-B radiation.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-10873663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-11270487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-11710930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-12517794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-12858333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-12908448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-15042383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-15664501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-16886686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-17170736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-17483180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-2423114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-2451669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-6196178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-8648185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-9747657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20592798-9927298
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1938-1980
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
1
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
239-45
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| pubmed:year |
2009
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| pubmed:articleTitle |
1,25-dihydroxyvitamin D(3) protects human keratinocytes against UV-B-induced damage: In vitro analysis of cell viability/proliferation, DNA-damage and -repair.
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| pubmed:affiliation |
Department of Dermatology; The Saarland University Hospital; Homburg/Saar, Germany.
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| pubmed:publicationType |
Journal Article
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