Source:http://linkedlifedata.com/resource/pubmed/id/20591502
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3-4
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pubmed:dateCreated |
2010-9-6
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pubmed:abstractText |
Interleukin 4 (IL-4) plays a central role in immune responses to parasites and allergens. IL-4 drives the differentiation of naive T cells into Th2 cells and regulates immunoglobulin class switching to IgE.Little is known about the role of IL-4 in canine allergies and parasite infections. Most of the information derives from measurement of IL-4 mRNA expression in dog tissues, but detection of IL-4 protein has been difficult so far, probably due to low sensitivity of available methods. Antibodies (Ab) specific for canine IL-4 are available from various sources, but these Ab have been produced against recombinant Escherichia coli-expressed canine IL-4 and there is only limited information on their reactivities with native canine IL-4. Therefore, in the present study, we tested six available canine IL-4-specific Ab for their reactivities with recombinant canine IL-4 expressed in E. coli (rec.IL-4) or in mammalian cells (mam.IL-4), and with supernatants from stimulated canine peripheral blood mononuclear cells (PBMCs) using several detection methods, including Western blotting, ELISA, cytokine bead assay, and intracellular IL-4 staining. Additionally, we tested a bovine IL-4-specific antibody that has been previously shown to cross-react with canine IL-4. All tested Ab except anti-bovine IL-4 reacted with rec.IL-4, and most of them reacted with mam.IL-4. However, only the cytokine bead assay was sensitive enough to allow the detection of IL-4 in supernatants of canine PBMCs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1873-2534
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pubmed:author | |
pubmed:copyrightInfo |
(c) 2010 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
310-6
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pubmed:meshHeading |
pubmed-meshheading:20591502-Animals,
pubmed-meshheading:20591502-Antibodies,
pubmed-meshheading:20591502-Blotting, Western,
pubmed-meshheading:20591502-Cell Line,
pubmed-meshheading:20591502-Dogs,
pubmed-meshheading:20591502-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:20591502-Flow Cytometry,
pubmed-meshheading:20591502-Humans,
pubmed-meshheading:20591502-Interleukin-4,
pubmed-meshheading:20591502-Recombinant Proteins
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pubmed:year |
2010
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pubmed:articleTitle |
Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays.
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pubmed:affiliation |
Department of Clinical Research, Vetsuisse Faculty, University of Bern, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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