Linked Life Data

20570723

Source:http://linkedlifedata.com/resource/pubmed/id/20570723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-7-13
pubmed:abstractText
In epilepsy, traumatic brain injury, and ischemic stroke, toxic levels of zinc released from neurons contribute to the brain damage associated with these disorders. Zinc causes oxidative stress by increasing the generation of reactive oxygen species and by inhibiting glutathione reductase (GR). This study investigated whether naturally occurring amino acids can protect cultured mouse astrocytes from zinc. Astrocytes incubated for 24h with 33 microM zinc acetate in a minimal medium displayed a loss of GR activity, a depletion of total glutathione, and a loss of total antioxidant capacity. These changes were accompanied by extensive cell death. Four amino acids (200 microM L-histidine, 201 microM L-cystine, 4mM L-glutamine, 798 microM L-threonine), which are a subset of the 15 present in Dulbecco's modified Eagle medium, were found to collectively prevent zinc toxicity. Histidine was the most protective, followed by cystine, glutamine, and threonine. It is proposed that each of these amino acids protects against different aspects of zinc toxicity by chelating zinc, by serving as precursors for glutathione, or by converting to tricarboxylic acid cycle intermediates. It is possible that these 4 amino acids contribute in vivo to the protection of brain cells from the toxic effects of zinc.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1873-4596
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
649-57
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Histidine, cystine, glutamine, and threonine collectively protect astrocytes from the toxicity of zinc.
pubmed:affiliation
Blood-Brain Interactions Group, School of Psychology & Psychiatry, Monash University, Clayton, VIC 3800, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't