|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2011-1-14
|
|
pubmed:abstractText |
In the subependymal zone and the dentate gyrus of the adult brain of rodents, neural stem cells with glial properties generate new neurons in a life-long process. The identification of glial progenitors outside the neurogenic niches, oligodendrocyte precursors in the healthy brain, and reactive astrocytes after cortical injury led to the idea of using these cells as endogenous cell source for neural repair in the cerebral cortex. Recently, our group showed that proliferating astroglia from the cerebral cortex can be reprogrammed into neurons capable of action potential firing by forced expression of neurogenic fate determinants but failed to develop synapses. Here, we describe a maturation profile of cultured reprogrammed NG2+ and glial fibrillary acidic protein+ glia cells of the postnatal rat cortex that ends with the establishment of a glutamatergic neuronal network. Within 3 weeks after viral expression of the transcription factor neurogenin 2 (Ngn2), glia-derived neurons exhibit network-driven, glutamate receptor-dependent oscillations in Ca(2+) and exhibit functional pre- and postsynaptic specialization. Interestingly, the Ngn2-instructed glutamatergic network also supports the maturation of a ?-aminobutyric acid (GABA)ergic input via GABA(A) receptors in a non-cell autonomous manner. The "proof-of-principle" results imply that a single transcription factor may be sufficient to instruct a neuronal network from a glia-like cell source.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/Bicuculline,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/GABA-A Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/GABA-A Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscimol,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Ngn2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, GABA-A,
http://linkedlifedata.com/resource/pubmed/chemical/Shank1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin,
http://linkedlifedata.com/resource/pubmed/chemical/beta3 tubulin, rat,
http://linkedlifedata.com/resource/pubmed/chemical/doublecortin protein
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
1460-2199
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
21
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
413-24
|
|
pubmed:dateRevised |
2011-6-29
|
|
pubmed:meshHeading |
pubmed-meshheading:20562320-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:20562320-Age Factors,
pubmed-meshheading:20562320-Animals,
pubmed-meshheading:20562320-Animals, Newborn,
pubmed-meshheading:20562320-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:20562320-Bicuculline,
pubmed-meshheading:20562320-Calcium,
pubmed-meshheading:20562320-Cell Differentiation,
pubmed-meshheading:20562320-Cells, Cultured,
pubmed-meshheading:20562320-Cerebral Cortex,
pubmed-meshheading:20562320-GABA-A Receptor Agonists,
pubmed-meshheading:20562320-GABA-A Receptor Antagonists,
pubmed-meshheading:20562320-Green Fluorescent Proteins,
pubmed-meshheading:20562320-Microtubule-Associated Proteins,
pubmed-meshheading:20562320-Muscimol,
pubmed-meshheading:20562320-Nerve Net,
pubmed-meshheading:20562320-Nerve Tissue Proteins,
pubmed-meshheading:20562320-Neuroglia,
pubmed-meshheading:20562320-Neurons,
pubmed-meshheading:20562320-Neuropeptides,
pubmed-meshheading:20562320-Patch-Clamp Techniques,
pubmed-meshheading:20562320-Rats,
pubmed-meshheading:20562320-Receptors, GABA-A,
pubmed-meshheading:20562320-Retroviridae,
pubmed-meshheading:20562320-Sodium Channel Blockers,
pubmed-meshheading:20562320-Synaptic Transmission,
pubmed-meshheading:20562320-Tetrodotoxin,
pubmed-meshheading:20562320-Transduction, Genetic,
pubmed-meshheading:20562320-Tubulin
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Neuronal network formation from reprogrammed early postnatal rat cortical glial cells.
|
|
pubmed:affiliation |
Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, 80336 Munich, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
