Linked Life Data

20553800

Source:http://linkedlifedata.com/resource/pubmed/id/20553800

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-7-13
pubmed:abstractText
Micronuclear genes in stichotrichous ciliates are broken into blocks separated by noncoding sequences, sometimes with the blocks in a shuffled order, some even inverted. During reproduction, all blocks are assembled in the correct order and orientation. This process is possible due to the special structure of micronuclear genes: each coding block M ends with a short nucleotide sequence (called pointer) that is repeated at the beginning of the coding block that should follow M in the assembled gene. Many of the pointers have multiple occurrences along both strands of the gene. This yields a very high number of pointer-induced possible divisions into coding and noncoding blocks. We investigate the distribution of pointers for all currently sequenced micronuclear ciliate genes with the goal of identifying what distinguishes the real gene structure among all possible coding/noncoding divisions. We find a sharp criterion in the average a/t-content of the noncoding blocks: the real division has, in most cases, the maximum such content among all possible combinations. Even for pointers as short as two nucleotides, the real division is one of very few with an average a/t-content of its noncoding blocks over 80%. The separation is most clear when the loci of pointers of up to four nucleotides (even three in the case of unscrambled genes) are fixed (e.g., through a template-based recombination mechanism).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1872-8324
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-16
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
A sequence-based analysis of the pointer distribution of stichotrichous ciliates.
pubmed:affiliation
Laboratoire d'Algorithmique, Complexité et Logique, Département Informatique, Université Paris Est, 61 av. Général de Gaulle, Créteil, France. verlan@univ-paris12.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't