| pubmed-article:20544254 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20544254 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:20544254 | lifeskim:mentions | umls-concept:C0315191 | lld:lifeskim |
| pubmed-article:20544254 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:20544254 | lifeskim:mentions | umls-concept:C0017372 | lld:lifeskim |
| pubmed-article:20544254 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:20544254 | pubmed:dateCreated | 2010-10-18 | lld:pubmed |
| pubmed-article:20544254 | pubmed:abstractText | We recently proposed a metabolic engineering strategy for L-ornithine production based on the hypothesis that an increased intracellular supply of N-acetylglutamate may further enhance L-ornithine production in a well-defined recombinant strain of Corynebacterium glutamicum. In this work, an argJ-deficient arginine auxotrophic mutant of C. glutamicum is suppressed by a different locus of C. glutamicum ATCC13032. Overexpression of the NCgl1469 open reading frame (ORF), exhibiting N-acetylglutamate synthase (NAGS) activity, was able to complement the C. glutamicum arginine-auxotrophic argJ strain and showed increased NAGS activity from 0.03 to 0.17 units mg(-1) protein. Additionally, overexpression of the NCgl1469 ORF resulted in a 39% increase in excreted L-ornithine. These results indicate that the intracellular supply of N-acetylglutamate is a rate-limiting step during L-ornithine production in C. glutamicum. | lld:pubmed |
| pubmed-article:20544254 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20544254 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20544254 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20544254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20544254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20544254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20544254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20544254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20544254 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20544254 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20544254 | pubmed:issn | 1476-5535 | lld:pubmed |
| pubmed-article:20544254 | pubmed:author | pubmed-author:ChoJae-YongJY | lld:pubmed |
| pubmed-article:20544254 | pubmed:author | pubmed-author:HwangGui-HyeG... | lld:pubmed |
| pubmed-article:20544254 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20544254 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:20544254 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20544254 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20544254 | pubmed:pagination | 1131-6 | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:meshHeading | pubmed-meshheading:20544254... | lld:pubmed |
| pubmed-article:20544254 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20544254 | pubmed:articleTitle | Identification of a suppressor gene for the arginine-auxotrophic argJ mutation in Corynebacterium glutamicum. | lld:pubmed |
| pubmed-article:20544254 | pubmed:affiliation | Department of Pharmaceutical Engineering, College of Health Science, Sangji University, 660 Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea. | lld:pubmed |
| pubmed-article:20544254 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20544254 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:1019371 | entrezgene:pubmed | pubmed-article:20544254 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20544254 | lld:entrezgene |
