Linked Life Data

20544254

Source:http://linkedlifedata.com/resource/pubmed/id/20544254

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-10-18
pubmed:abstractText
We recently proposed a metabolic engineering strategy for L-ornithine production based on the hypothesis that an increased intracellular supply of N-acetylglutamate may further enhance L-ornithine production in a well-defined recombinant strain of Corynebacterium glutamicum. In this work, an argJ-deficient arginine auxotrophic mutant of C. glutamicum is suppressed by a different locus of C. glutamicum ATCC13032. Overexpression of the NCgl1469 open reading frame (ORF), exhibiting N-acetylglutamate synthase (NAGS) activity, was able to complement the C. glutamicum arginine-auxotrophic argJ strain and showed increased NAGS activity from 0.03 to 0.17 units mg(-1) protein. Additionally, overexpression of the NCgl1469 ORF resulted in a 39% increase in excreted L-ornithine. These results indicate that the intracellular supply of N-acetylglutamate is a rate-limiting step during L-ornithine production in C. glutamicum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1476-5535
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1131-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Identification of a suppressor gene for the arginine-auxotrophic argJ mutation in Corynebacterium glutamicum.
pubmed:affiliation
Department of Pharmaceutical Engineering, College of Health Science, Sangji University, 660 Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't