Linked Life Data

20542131

Source:http://linkedlifedata.com/resource/pubmed/id/20542131

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-7-26
pubmed:databankReference
pubmed:abstractText
The sliding clamp proliferating cell nuclear antigen (PCNA) plays important roles in nucleic acid metabolism. In this work, we isolated a PCNA gene (designated as EjsPCNA, accession: FJ483830) by rapid amplification of cDNA ends approach from the Chinese mitten crab Eriocheir japonica sinensis. The full-length cDNA of EjsPCNA consists of 1123 nucleotides with an open-reading frame of 780bp encoding 259 amino acids (28.62kDa) and containing an interdomain connecting loop, C-terminal tail, and center loop. Sequence alignment, phylogenetic analyses, and structure comparison revealed that EjsPCNA is a member of the PCNA family. Real-time RT-PCR results indicate that EjsPCNA is expressed throughout three developmental stages. EjsPCNA mRNA expression levels at the first crab stage are significantly higher than that of the other two stages. Present data showed that the expression levels of EjsPCNA in E. j. sinensis are likely related to proliferation activity of tissues, and suggested that EjsPCNA gene is probably involved in the crabs' early developmental regulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1531-4332
pubmed:author
pubmed:copyrightInfo
2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
170-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Identification, mRNA expression and characterization of proliferating cell nuclear antigen gene from Chinese mitten crab Eriocheir japonica sinensis.
pubmed:affiliation
Nanjing Normal University, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't