Linked Life Data

2054103

Source:http://linkedlifedata.com/resource/pubmed/id/2054103

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-7-29
pubmed:abstractText
Determination of amino acid replacements or assessment of sequence features localized in internal regions of natural or genetically engineered proteins can be performed in some cases with the expenditure of a minimum amount of work and protein material. The procedure requires fragmentation of a protein sample by a chemical or enzymatic method: one of the fragments should include the sequence tract under investigation, suitably preceded by one or more prolyl residues. Classical Edman degradation can be performed on the whole mixture of fragments, thus avoiding the rate-limiting step of peptide purification. Sequence data become readable after performance of reaction with o-phthalaldehyde at the level of proline residues in the relevant peptide. Two specific cases illustrate the potentiality of the procedure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0885-4513
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
48-53
pubmed:dateRevised
2008-7-12
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Assessment of sequence features in internal regions of proteins.
pubmed:affiliation
Dipartimento di Scienze Biochimiche, Università La Sapienza, Rome, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't