Linked Life Data

20516187

Source:http://linkedlifedata.com/resource/pubmed/id/20516187

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-6-2
pubmed:abstractText
In this protocol, active anaphase promoting complex/cyclosome (APC/C) and its inhibitor, mitotic checkpoint complex (MCC), are purified from HeLa cell extracts. The two complexes are first separated by gel filtration and ion exchange chromatography. The MCC is further purified by an additional gel filtration step. The APC/C activity and the ability of MCC to inhibit the APC/C are assayed at every stage of the purification procedure by in vitro ubiquitination assays. The composition and distribution of the complexes are monitored by Western blot analysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1559-6095
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2010
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
pdb.prot5449
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Purification of the mitotic checkpoint complex (MCC) and the anaphase promoting complex/cyclosome (APC/C) from HeLa cells.
pubmed:publicationType
Journal Article