Source:http://linkedlifedata.com/resource/pubmed/id/20509218
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2010-5-28
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pubmed:abstractText |
l. In the hepatopancreas of the shrimp Penaeus japonicus the beta-glucosidase is present, either free or membrane-bound. The specific activity of the purified enzyme is 237,333 units/mg of protein and 191,111 units/mg of protein for the free and the membrane-bound beta-glucosidase, respectively. 2.The non-membrane-bound beta-glucosidase appears to be the same molecular size as the membrane-bound enzyme, both being monomers and consisting of a polypeptide chain of apparent M(r) 65,000, as estimated by chromatography on Superose 12 and by SDS-PAGE. 3.Both enzymes share similarities in their molecular size and substrate specificities (with ratios of 100:17:4:12 for the non-membrane-bound and 100:32:13:12 for the membrane-bound enzyme for the activities with methylumbelliferyl-beta-D-glucoside, methylumbelliferyl-beta-D-galactoside, methylumbelliferyl-alpha -L-arabinopyranoside and methylumbelliferyl-beta-xyloside respectively, as substrates). 4.The membrane-bound beta-glucosidase can be differentiated from the non-membrane-bound enzyme by its isoelectric point (7.5 vs 6.6), Km (182 micrometer vs 76 micrometer), pH optimum (4.5 vs 5.5), phosphorylation,sialyation and thermostability.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-0491
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
279-83
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pubmed:meshHeading |
pubmed-meshheading:20509218-Animals,
pubmed-meshheading:20509218-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:20509218-Hepatopancreas,
pubmed-meshheading:20509218-Hot Temperature,
pubmed-meshheading:20509218-Hydrogen-Ion Concentration,
pubmed-meshheading:20509218-Isoelectric Focusing,
pubmed-meshheading:20509218-Membrane Proteins,
pubmed-meshheading:20509218-Molecular Weight,
pubmed-meshheading:20509218-Penaeidae,
pubmed-meshheading:20509218-Phosphorylation,
pubmed-meshheading:20509218-Substrate Specificity,
pubmed-meshheading:20509218-Time Factors,
pubmed-meshheading:20509218-beta-Glucosidase
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pubmed:year |
1992
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pubmed:articleTitle |
Comparative study of free and membrane-bound acidic beta-D-glucosidase from the hepatopancreas of the shrimp Penaeus japonicus (Crustacea: decapoda).
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pubmed:affiliation |
Division of Biochemistry and Molecular Science, Institute of Zoology, Academia Sinica, Nankang, Taipei,Republic of China.
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pubmed:publicationType |
Journal Article,
Comparative Study
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