Linked Life Data

20491546

Source:http://linkedlifedata.com/resource/pubmed/id/20491546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-6-16
pubmed:abstractText
Winter cold hardiness of insects typically involves one of two major strategies for survival below 0 degrees C: freeze avoidance and freeze tolerance. The two strategies have some common features, including the accumulation of high concentrations of cryoprotectant polyols and the frequent occurrence of diapause. Entry into the hypometabolic state of diapause requires coordinated suppression of major ATP-consuming metabolic processes, and ion motive ATPases are important targets for regulation. This study documents the suppression of sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) activity in the overwintering larvae of two cold-hardy species, the freeze-avoiding gall moth Epiblema scudderiana and the freeze-tolerant gall fly Eurosta solidaginis. Activity was reduced despite a lack of change in SERCA protein levels in E. solidaginis larvae over the winter and a six- to eightfold increase in SERCA protein in E. scudderiana. This implicated posttranslational modification as the mechanism of SERCA suppression, and in vitro incubations indicated that enzyme phosphorylation by protein kinases A, G, or C strongly reduced enzyme activity. A stable reduction in SERCA activity was also seen in cold-acclimated larvae of both species compared with 15 degrees C controls, with significant changes in the kinetic parameters of the E. scudderiana enzyme (e.g., K(m) ATP was 3.2-fold higher in -20 degrees C-acclimated larvae) that were consistent with reduced enzyme function at low temperature. Epiblema scudderiana SERCA was also subject to regulation by differential temperature effects (Arrhenius activation energy increased by approximately threefold below 10 degrees C) and by seasonal changes in the levels of a SERCA inhibitor protein, phospholamban.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1537-5293
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
677-86
pubmed:meshHeading
pubmed:articleTitle
In cold-hardy insects, seasonal, temperature, and reversible phosphorylation controls regulate sarco/endoplasmic reticulum Ca2+-ATPase (SERCA).
pubmed:affiliation
Institute of Biochemistry and Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada. david.mcmullen.ctr@usuhs.mil
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't