Linked Life Data

20484639

Source:http://linkedlifedata.com/resource/pubmed/id/20484639

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2010-5-20
pubmed:abstractText
Midbrain dopamine neurons release dopamine from both axons and dendrites. The mechanism underlying release at these different sites has been proposed to differ. This study used electrochemical and electrophysiological methods to compare the time course and calcium dependence of somatodendritic dopamine release in the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc) to that of axonal dopamine release in the dorsal striatum. The amount of dopamine released in the striatum was approximately 20-fold greater than in cell body regions of the VTA or SNc. However, the calcium dependence and time to peak of the dopamine transients were similar. These results illustrate an unexpected overall similarity in the mechanisms of dopamine release in the striatum and cell body regions. To examine how diffusion regulates the time course of dopamine following release, dextran was added to the extracellular solution to slow diffusion. In the VTA, dextran slowed the rate of rise and fall of the extracellular dopamine transient as measured by fast-scan cyclic voltammetry yet did not alter the kinetics of the dopamine-dependent IPSC. Dextran failed to significantly alter the time course of the rise and fall of the dopamine transient in the striatum, suggesting a more influential role for reuptake in the striatum. The conclusion is that the time course of dopamine within the extracellular space of the VTA is dependent on both diffusion and reuptake, whereas the activation of D(2) receptors on dopamine neurons is primarily limited by reuptake.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
19
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6975-83
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed-meshheading:20484639-Analysis of Variance, pubmed-meshheading:20484639-Animals, pubmed-meshheading:20484639-Axons, pubmed-meshheading:20484639-Biophysics, pubmed-meshheading:20484639-Calcium, pubmed-meshheading:20484639-Dendrites, pubmed-meshheading:20484639-Dextrans, pubmed-meshheading:20484639-Dopamine, pubmed-meshheading:20484639-Electric Stimulation, pubmed-meshheading:20484639-Electrochemistry, pubmed-meshheading:20484639-Female, pubmed-meshheading:20484639-GABA Antagonists, pubmed-meshheading:20484639-Indoles, pubmed-meshheading:20484639-Inhibitory Postsynaptic Potentials, pubmed-meshheading:20484639-Iontophoresis, pubmed-meshheading:20484639-Male, pubmed-meshheading:20484639-Mice, pubmed-meshheading:20484639-Mice, Inbred DBA, pubmed-meshheading:20484639-Neurons, pubmed-meshheading:20484639-Patch-Clamp Techniques, pubmed-meshheading:20484639-Phosphinic Acids, pubmed-meshheading:20484639-Propanolamines, pubmed-meshheading:20484639-Serotonin, pubmed-meshheading:20484639-Substantia Nigra, pubmed-meshheading:20484639-Time Factors, pubmed-meshheading:20484639-Ventral Tegmental Area
pubmed:year
2010
pubmed:articleTitle
Control of extracellular dopamine at dendrite and axon terminals.
pubmed:affiliation
Vollum Institute, Oregon Health & Science University L474, Portland, Oregon 97239, USA. fordc@ohsu.edu
pubmed:publicationType
Journal Article, In Vitro, Research Support, N.I.H., Extramural