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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2010-6-10
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pubmed:abstractText |
The foxa regulatory gene is of central importance for endoderm specification across Bilateria, and this gene lies at an essential node of the well-characterized sea urchin endomesoderm gene regulatory network (GRN). Here we experimentally dissect the cis-regulatory system that controls the complex pattern of foxa expression in these embryos. Four separate cis-regulatory modules (CRMs) cooperate to control foxa expression in different spatial domains of the endomesoderm, and at different times. A detailed mutational analysis revealed the inputs to each of these cis-regulatory modules. The complex and dynamic expression of foxa is regulated by a combination of repressors, a permissive switch, and multiple activators. A mathematical kinetic model was applied to study the dynamic response of foxa cis-regulatory modules to transient inputs. This study shed light on the mesoderm-endoderm fate decision and provides a functional explanation, in terms of the genomic regulatory code, for the spatial and temporal expression of a key developmental control gene.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
107
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10103-8
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pubmed:dateRevised |
2011-3-3
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pubmed:meshHeading |
pubmed-meshheading:20479235-Animals,
pubmed-meshheading:20479235-Animals, Genetically Modified,
pubmed-meshheading:20479235-Base Sequence,
pubmed-meshheading:20479235-Binding Sites,
pubmed-meshheading:20479235-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:20479235-DNA,
pubmed-meshheading:20479235-Endoderm,
pubmed-meshheading:20479235-Forkhead Transcription Factors,
pubmed-meshheading:20479235-Gene Expression Regulation, Developmental,
pubmed-meshheading:20479235-Gene Regulatory Networks,
pubmed-meshheading:20479235-Genetic Complementation Test,
pubmed-meshheading:20479235-Green Fluorescent Proteins,
pubmed-meshheading:20479235-Kinetics,
pubmed-meshheading:20479235-Mesoderm,
pubmed-meshheading:20479235-Models, Biological,
pubmed-meshheading:20479235-Molecular Sequence Data,
pubmed-meshheading:20479235-Mutation,
pubmed-meshheading:20479235-RNA, Messenger,
pubmed-meshheading:20479235-Recombinant Proteins,
pubmed-meshheading:20479235-Strongylocentrotus purpuratus
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pubmed:year |
2010
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pubmed:articleTitle |
Information processing at the foxa node of the sea urchin endomesoderm specification network.
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pubmed:affiliation |
Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA. smadar@caltech.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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