Linked Life Data

20473940

Source:http://linkedlifedata.com/resource/pubmed/id/20473940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-1-31
pubmed:abstractText
miRNAs have emerged as post-transcriptional regulators that are critically involved in the pathogenesis of a number of human cancers. Cdc42, one of the best characterized members of the Rho GTPase family, is found to be up-regulated in several types of human tumors and has been implicated in cancer initiation and progression. In the present study, we have identified miR-137 as a potential regulator of Cdc42 expression. A bioinformatics search revealed a putative target-site for miR-137 within the Cdc42 3' UTR at nt 792-798, which is highly conserved across different species. Expression of miR-137 in colorectal cancer cell lines was found inversely correlated with Cdc42 expression. miR-137 could significantly suppress Cdc42 3' UTR luciferase-reporter activity, and this effect was not detectable when the putative 3' UTR target-site was mutated. Consistent with the results of the reporter assay, ectopic expression of miR-137 reduced both mRNA and protein expression levels of Cdc42 and mimicked the effect of Cdc42 knockdown in inhibiting proliferation, inducing G1 cell cycle arrest, and blocking invasion of the colorectal cancer cells, whereas anti-miR-137 expression led to the opposite effect. Furthermore, expression of miR-137 suppressed the immediate downstream effector of Cdc42, PAK signaling. Our results suggest that miR-137 may have a tumor suppressor function by directly targeting Cdc42 to inhibit the proliferation and invasion activities of colorectal cancer cells. They raise an interesting possibility that Cdc42 activity and function can be controlled by miRNAs in addition to the classic regulators such as guanine nucleotide exchange factors and GTPase-activating proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1097-0215
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 UICC.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1269-79
pubmed:meshHeading
pubmed-meshheading:20473940-3' Untranslated Regions, pubmed-meshheading:20473940-Apoptosis, pubmed-meshheading:20473940-Blotting, Western, pubmed-meshheading:20473940-Cell Adhesion, pubmed-meshheading:20473940-Cell Line, Tumor, pubmed-meshheading:20473940-Cell Movement, pubmed-meshheading:20473940-Cell Proliferation, pubmed-meshheading:20473940-Collagen, pubmed-meshheading:20473940-Colorectal Neoplasms, pubmed-meshheading:20473940-Drug Combinations, pubmed-meshheading:20473940-G1 Phase, pubmed-meshheading:20473940-Gene Expression Regulation, Neoplastic, pubmed-meshheading:20473940-Humans, pubmed-meshheading:20473940-Laminin, pubmed-meshheading:20473940-Luciferases, pubmed-meshheading:20473940-MicroRNAs, pubmed-meshheading:20473940-Proteoglycans, pubmed-meshheading:20473940-RNA, Messenger, pubmed-meshheading:20473940-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20473940-cdc42 GTP-Binding Protein
pubmed:year
2011
pubmed:articleTitle
miR-137 targets Cdc42 expression, induces cell cycle G1 arrest and inhibits invasion in colorectal cancer cells.
pubmed:affiliation
Laboratory of Signal Transduction & Molecular Targeted Therapy, State Key Laboratory of Biotherapy/Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't