Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-6-14
pubmed:abstractText
Sildenafil is the first oral PDE5 inhibitor for the treatment of erectile dysfunction and pulmonary arterial hypertension. In the present study, we investigated the effect of sildenafil on adipogenesis in 3T3L1 preadipocytes. Treatment with sildenafil for 8 days significantly promoted adipogenesis characterized by increased lipid droplet and triglyceride content in 3T3L1 cells. Meanwhile, sildenafil induced a pronounced up-regulation of the expression of adipocyte-specific genes, such as aP2 and GLUT4. The results by RT-PCR and Western blotting further showed that sildenafil increased the sequential expression of C/EBP beta, PPAR gamma and C/EBP alpha. Additionally, we found that the other two PDE5 inhibitors (vardenafil and tadalafil) and the cGMP analog 8-pCPT-cGMP also increased adipogenesis. Likewise, 8-pCPT-cGMP could up-regulate the expression of adipogenic and adipocyte-specific genes. Importantly, the PKG inhibitor Rp-8-pCPT-cGMP was able to inhibit both sildenafil and 8-pCPT-cGMP-induced adipogenesis. Furthermore, sildenafil promoted basal and insulin-mediated glucose uptake in 3T3L1 cells, which was counteracted by Rp-8-pCPT-cGMP. These results indicate that sildenafil could promote adipogenesis accompanied by increased glucose uptake through a PKG pathway at least partly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/8-(4-chlorophenylthio)guanosine..., http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-beta, http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/CEBPA protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Cebpb protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP-Dependent Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Fabp4 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acid-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glucose, http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/PPAR gamma, http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase 5 Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Piperazines, http://linkedlifedata.com/resource/pubmed/chemical/Purines, http://linkedlifedata.com/resource/pubmed/chemical/Sulfones, http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides, http://linkedlifedata.com/resource/pubmed/chemical/sildenafil
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1090-2104
pubmed:author
pubmed:copyrightInfo
(c) 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
11
pubmed:volume
396
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1054-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:20471953-3T3-L1 Cells, pubmed-meshheading:20471953-Adipocytes, pubmed-meshheading:20471953-Adipogenesis, pubmed-meshheading:20471953-Animals, pubmed-meshheading:20471953-CCAAT-Enhancer-Binding Protein-beta, pubmed-meshheading:20471953-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:20471953-Cyclic GMP, pubmed-meshheading:20471953-Cyclic GMP-Dependent Protein Kinases, pubmed-meshheading:20471953-Fatty Acid-Binding Proteins, pubmed-meshheading:20471953-Gene Expression, pubmed-meshheading:20471953-Glucose, pubmed-meshheading:20471953-Glucose Transporter Type 4, pubmed-meshheading:20471953-Insulin, pubmed-meshheading:20471953-Mice, pubmed-meshheading:20471953-PPAR gamma, pubmed-meshheading:20471953-Phosphodiesterase 5 Inhibitors, pubmed-meshheading:20471953-Phosphodiesterase Inhibitors, pubmed-meshheading:20471953-Piperazines, pubmed-meshheading:20471953-Purines, pubmed-meshheading:20471953-Sulfones, pubmed-meshheading:20471953-Thionucleotides
pubmed:year
2010
pubmed:articleTitle
Sildenafil promotes adipogenesis through a PKG pathway.
pubmed:affiliation
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhang Jiang Hi-Tech Park, Pudong, Shanghai 201203, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't