Source:http://linkedlifedata.com/resource/pubmed/id/20471275
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2010-5-31
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pubmed:abstractText |
Heat-shock protein 47 (HSP47) is a chaperone that facilitates the proper folding of procollagen. Our previous studies showed that the high-affinity HSP47-binding motif in the collagen triple helix is Xaa-(Thr/Pro)-Gly-Xaa-Arg-Gly. In this study, we further investigated structural requirements for the HSP47-binding motif, using synthetic triple-helical collagen-model peptides with systematic amino acid substitutions at either the Thr/Pro (=Yaa(-3)) or the Arg (=Yaa(0)) position. Results obtained from in vitro binding assays indicated that HSP47 detects the side-chain structure of Arg at the Yaa(0)-position, while the Yaa(-3) amino acid serves as the secondary recognition site that affects affinity to HSP47.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1464-3391
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3767-75
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pubmed:meshHeading |
pubmed-meshheading:20471275-Amino Acid Sequence,
pubmed-meshheading:20471275-Animals,
pubmed-meshheading:20471275-Binding Sites,
pubmed-meshheading:20471275-Collagen,
pubmed-meshheading:20471275-HSP47 Heat-Shock Proteins,
pubmed-meshheading:20471275-Mice,
pubmed-meshheading:20471275-Protein Binding,
pubmed-meshheading:20471275-Structure-Activity Relationship
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pubmed:year |
2010
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pubmed:articleTitle |
A structure-activity relationship study elucidating the mechanism of sequence-specific collagen recognition by the chaperone HSP47.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, Waseda University, Tokyo 169-8555, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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