Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.
Centre National de la Recherche Scientifique, IFR88, UPR 9043, Laboratoire de Chimie Bactérienne, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France.
