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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2010-10-18
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pubmed:abstractText |
The underlying mechanism of palmitate-induced insulin resistance in skeletal muscle cells is obscure. In this study, we showed that palmitate inhibited the insulin signaling in C2C12 myotubes, accompanied with the enhanced phosphorylation of protein kinase C-theta (PKC?). The inhibitory effects of palmitate on the insulin signaling were diminished in PKC?- and mTOR (mammalian target of rapamycin)-deficient C2C12 myotubes, and C2C12 myotubes pre-treated with rapamycin. In addition, the phosphorylation of mTOR and p70 ribosomal S6 kinase (S6K) enhanced by palmitate was attenuated in PKC?-deficient C2C12 myotubes and in C2C12 myotubes treated with PKC? pseudosubstrate. Taken together, our results suggested that palmitate-induced insulin resistance in C2C12 myotubes is mediated by PKC?/mTOR/S6K pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1439-3646
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pubmed:author |
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pubmed:copyrightInfo |
© Georg Thieme Verlag KG Stuttgart · New York.
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pubmed:issnType |
Electronic
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
657-61
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pubmed:meshHeading |
pubmed-meshheading:20429048-Animals,
pubmed-meshheading:20429048-Cells, Cultured,
pubmed-meshheading:20429048-Enzyme Activation,
pubmed-meshheading:20429048-Insulin Resistance,
pubmed-meshheading:20429048-Isoenzymes,
pubmed-meshheading:20429048-Mice,
pubmed-meshheading:20429048-Muscle Fibers, Skeletal,
pubmed-meshheading:20429048-Palmitic Acid,
pubmed-meshheading:20429048-Phosphorylation,
pubmed-meshheading:20429048-Protein Kinase C,
pubmed-meshheading:20429048-Ribosomal Protein S6 Kinases,
pubmed-meshheading:20429048-Signal Transduction,
pubmed-meshheading:20429048-TOR Serine-Threonine Kinases,
pubmed-meshheading:20429048-Up-Regulation
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pubmed:year |
2010
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pubmed:articleTitle |
Palmitate induced insulin resistance by PKCtheta-dependent activation of mTOR/S6K pathway in C2C12 myotubes.
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pubmed:affiliation |
Department of Pathophysiology, Yunyang Medical College, Shiyan, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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