Linked Life Data

20413890

Source:http://linkedlifedata.com/resource/pubmed/id/20413890

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-7-5
pubmed:abstractText
Neuroscience, including research on Alzheimer's disease, is hampered by the lack of suitable in vitro models to study the human nervous system. To counteract this, many attempts to differentiate cell lines into more neuron-like cells have been performed, resulting in partial expression of neuronal features. Furthermore, it has been reported that neuroblastoma cell lines lack mature isoforms of tau. Our aim was to develop an improved in vitro model, generating sustainable cells with morphology and biochemistry of human, mature neurons. To obtain cells with neuronal differentiation and function, we investigated the effect of combining three-dimensional culturing of SH-SY5Y cells in extracellular matrix (ECM) gel with several factors reported to have neuro-differentiating effects. This resulted in cells with apparent neuronal morphology with long, extensively branched neurites. Further investigation revealed expression of several neurospecific markers including synapse protein Sv2 and nuclear marker NeuN, as well as the presence of synapses and axonal vesicle transport. In addition, these cells expressed mature tau isoforms, and tau protein expression was significantly increased compared to undifferentiated cells, reaching levels found in adult human brain. In conclusion, we found that pre-treatment with retinoic acid followed by ECM gel culturing in combination with brain derived neurotrophic factor, neuregulin beta1, nerve growth factor, and vitamin D3 treatment generated sustainable cells with unambiguous resemblance to adult neurons. These cells also expresses adult splicing forms of tau with neuronal localization, making this cellular in vitro model useful in many areas of neuroscience research, particularly the Alzheimer's disease field.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1875-8908
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1069-82
pubmed:meshHeading
pubmed-meshheading:20413890-Alzheimer Disease, pubmed-meshheading:20413890-Antigens, Nuclear, pubmed-meshheading:20413890-Blotting, Western, pubmed-meshheading:20413890-Cell Differentiation, pubmed-meshheading:20413890-Cell Line, Tumor, pubmed-meshheading:20413890-Cholecalciferol, pubmed-meshheading:20413890-Extracellular Matrix, pubmed-meshheading:20413890-Humans, pubmed-meshheading:20413890-Immunohistochemistry, pubmed-meshheading:20413890-Microscopy, Fluorescence, pubmed-meshheading:20413890-Models, Neurological, pubmed-meshheading:20413890-Nerve Growth Factors, pubmed-meshheading:20413890-Nerve Tissue Proteins, pubmed-meshheading:20413890-Neurons, pubmed-meshheading:20413890-Neurosciences, pubmed-meshheading:20413890-Phosphorylation, pubmed-meshheading:20413890-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20413890-Synapses, pubmed-meshheading:20413890-Vitamins, pubmed-meshheading:20413890-tau Proteins
pubmed:year
2010
pubmed:articleTitle
An in vitro model for neuroscience: differentiation of SH-SY5Y cells into cells with morphological and biochemical characteristics of mature neurons.
pubmed:affiliation
Department of Clinical and Experimental Medicine, Division ofGeriatrics, Faculty of Health Science, Linköping University, Linköping, Sweden. lotta.hellstrom@liu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't