Linked Life Data

20405157

Source:http://linkedlifedata.com/resource/pubmed/id/20405157

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-5-14
pubmed:abstractText
We report a free-energy-based algorithm to estimate the step size of processive molecular motors from noisy, experimental time position traces. In our approach, the problem of estimating step sizes reduces to the evaluation of the free energy of directed lattice polymers in a random potential. The present approach is Bayesian in spirit as we do not aim to determine the most likely underlying time trace but rather to determine the step size and stepping frequency that are most likely to yield the observed data. We test this method on synthetic data for the simple case of noisy traces with fixed underlying step size and Poissonian stepping statistics. We find that the present scheme can work at signal-to-noise levels that are about 40% worse than those where the best existing step detection methods fail. More importantly, the present approach yields a much more accurate estimate of the step size. Although we focus on the case of non-reversing walks with a single step size, we show that we can detect if this assumption is violated. In principle, the method can be extended to more complex stepping scenarios but we find that for noisy data, multi-parameter fits are not reliable.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1292-895X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
411-7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Free-energy-based method for step size detection of processive molecular motors.
pubmed:affiliation
Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't