Linked Life Data

20394602

Source:http://linkedlifedata.com/resource/pubmed/id/20394602

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-11-10
pubmed:abstractText
The decline in the reproductive efficiency of dairy cows, especially those with high producing potential, has become a challenging problem. In this study, a selective DNA pooling approach was applied to a cow population whose oocytes were fertilized and cultured to obtain phenotypic records of fertilization rate and blastocyst rate. Using a stringent 5% genome-wide significance level, 22 and five single nucleotide polymorphisms (SNPs) were found to be associated with fertilization rate and blastocyst rate, respectively. SNPs that showed significant association in selective DNA pooling were further evaluated by individual genotyping. Interestingly, the majority of the SNP associations were confirmed by individual genotyping, testifying to the effectiveness of selective DNA pooling using a high-density SNP genotyping array. This study is the first application of the selective DNA pooling approach using the BovineSNP50 array in cattle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1365-2052
pubmed:author
pubmed:copyrightInfo
© 2010 The Authors, Animal Genetics © 2010 Stichting International Foundation for Animal Genetics.
pubmed:issnType
Electronic
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
570-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
A genome-wide association study using selective DNA pooling identifies candidate markers for fertility in Holstein cattle.
pubmed:affiliation
Department of Dairy Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't