Source:http://linkedlifedata.com/resource/pubmed/id/20384789
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2010-6-11
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pubmed:abstractText |
Lepidoptera such as the domestic silkworm (Bombyx mori) produce proteins modified with unsialylated, mannose-rich moieties known as 'high mannose-type'N-glycans. However, we observed that, under intrinsic acetylglucosaminidase (GlcNAcase)-inhibited conditions, moth cells tend to synthesize different types of glycoform with sialic acid modification. To identify molecules essential to assemble Lepidoptera-specific N-glycans, we performed BLAST analysis on the silkworm genetic database and isolated the entire coding sequence of novel Bombyx GlcNAcase, BmGlcNAcase 2. This enzyme showed weak homology to currently known, lysosome-associated eukaryotic hexosaminidases, but it revealed remarkable similarity with recently reported glycosyl hydrolases of Spodoptera and Bombyx. Interestingly, BmGlcNAcase 2 was found to be expressed in embryos and in certain tissues of molting larvae (i.e. ovary, fat bodies, mid-intestine, skin), but not in pupae, suggesting its unique function in the carbohydrate metabolism of juvenile silkworm.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1365-2443
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
525-36
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pubmed:meshHeading |
pubmed-meshheading:20384789-Amino Acid Sequence,
pubmed-meshheading:20384789-Animals,
pubmed-meshheading:20384789-Bombyx,
pubmed-meshheading:20384789-Cloning, Molecular,
pubmed-meshheading:20384789-Gene Expression Profiling,
pubmed-meshheading:20384789-Insect Proteins,
pubmed-meshheading:20384789-Isoenzymes,
pubmed-meshheading:20384789-Molecular Sequence Data,
pubmed-meshheading:20384789-Phylogeny,
pubmed-meshheading:20384789-Sequence Alignment,
pubmed-meshheading:20384789-Sequence Homology, Amino Acid,
pubmed-meshheading:20384789-beta-N-Acetylhexosaminidases
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pubmed:year |
2010
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pubmed:articleTitle |
Molecular cloning and expression profile analysis of a novel beta-D-N-acetylhexosaminidase of domestic silkworm (Bombyx mori).
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pubmed:affiliation |
Advanced Biologicals, National Institute of Animal Health, NARO, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. takehiro@affrc.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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