Linked Life Data

20373513

Source:http://linkedlifedata.com/resource/pubmed/id/20373513

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-4-7
pubmed:abstractText
This unit provides a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag-seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing both the cost and time required to generate gene expression profiles. The ultra-high-throughput sequencing capability of the Illumina platform allows the cost-effective generation of libraries containing an average of 20 million tags, a 200-fold improvement over classical LongSAGE. Tag-seq has less sequence composition bias, leading to a better representation of AT-rich tag sequences, and allows a more accurate profiling of a subset of the transcriptome characterized by AT-rich genes expressed at levels below the threshold of detection of LongSAGE (Morrissy et al., 2009).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1934-8258
pubmed:author
pubmed:copyrightInfo
(c) 2010 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 11.11.1-36
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Digital gene expression by tag sequencing on the illumina genome analyzer.
pubmed:affiliation
BCCA Genome Sciences Centre, University of British Columbia, Vancouver, British Columbia, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't