|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
1991-6-26
|
|
pubmed:abstractText |
PRP11 is a gene that encodes an essential function for pre-messenger RNA (mRNA) processing in Saccharomyces cerevisiae. We have carried out a mutational study to locate essential and non-essential regions of the PRP11 protein. The existing temperature-sensitive (ts) mutation (prp11-1) was isolated from the chromosome of the original mutant and its position in the gene was determined. When the prp11-1 gene was transcribed from the GAL1 promoter, the overproduced protein was able to reverse the ts prp11-1 phenotype; this is compatible with the possibility that the defect in the prp11-1 gene product affects its binding to the spliceosome. Thirteen linker-insertion mutations were constructed. Only five (prp11-4, 11-6, 11-10, -13 and -14) resulted in a null phenotype. One of these became temperature-sensitive when the insertion was reduced in size from four (prp11-10) to two (prp11-15) amino acids. A sequence of ten amino acids of which also occurs in the human U1 small nuclear ribonucleoprotein particle (snRNP) A protein and the U2 snRNP B" protein, when deleted from PRP11, had no phenotype and thus appears to be nonessential for PRP11 function. However, a linker-insertion mutation (prp11-10) immediately adjacent to this region resulted in a null phenotype.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0026-8925
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
226
|
|
pubmed:geneSymbol |
PRP11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
277-82
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:2034220-Amino Acid Sequence,
pubmed-meshheading:2034220-Base Sequence,
pubmed-meshheading:2034220-DNA, Fungal,
pubmed-meshheading:2034220-Fungal Proteins,
pubmed-meshheading:2034220-Gene Expression Regulation, Fungal,
pubmed-meshheading:2034220-Genes, Fungal,
pubmed-meshheading:2034220-HeLa Cells,
pubmed-meshheading:2034220-Humans,
pubmed-meshheading:2034220-Molecular Sequence Data,
pubmed-meshheading:2034220-Mutagenesis, Insertional,
pubmed-meshheading:2034220-Mutagenesis, Site-Directed,
pubmed-meshheading:2034220-Phenotype,
pubmed-meshheading:2034220-RNA, Small Nuclear,
pubmed-meshheading:2034220-RNA Processing, Post-Transcriptional,
pubmed-meshheading:2034220-RNA-Binding Proteins,
pubmed-meshheading:2034220-Saccharomyces cerevisiae,
pubmed-meshheading:2034220-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:2034220-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2034220-Temperature
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Genetic studies of the PRP11 gene of Saccharomyces cerevisiae.
|
|
pubmed:affiliation |
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
