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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1991-6-11
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pubmed:abstractText |
A self complementary oligodeoxynucleotide dodecamer containing an achiral bridged 5'-phosphorothioate linkage 3'-O-P-S-5' has been prepared using the solid phase phosphoramidite procedure. For the incorporation of the phosphorothioate link we have used a 5'-(S-trityl)-mercapto-5'-deoxythymidine-3'-phosphoramidite. After coupling this building block the S-trityl group was removed by silver ions. The free thiol moiety was then coupled with a standard phosphoramidite in the presence of tetrazole. After oxidation of the 5'-phosphorothioite with iodine/water the synthesis was continued with standard building blocks up to the desired dodecamer. This backbone modified dodecamer can be cleaved selectively and quantitatively at the P-S bond by silver or mercuric ions under very mild conditions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-1694695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-2315041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-2771637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-2783174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-3375062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-3375070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-3601657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-3822836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-4011448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-5016030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-5423828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-5760868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-5776259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-5844464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2027751-775443
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1437-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1991
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pubmed:articleTitle |
Synthesis and selective cleavage of an oligodeoxynucleotide containing a bridged internucleotide 5'-phosphorothioate linkage.
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pubmed:affiliation |
Institut für Organische Chemie, Johann Wolfgang Goethe-Universität Frankfurt, FRG.
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pubmed:publicationType |
Journal Article
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