Linked Life Data

20235100

Source:http://linkedlifedata.com/resource/pubmed/id/20235100

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-3-17
pubmed:abstractText
Binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) initiates signal transduction, ultimately leading to altered gene expression. Ligand-activated EGFR is also rapidly internalized and then targeted to lysosomes for degradation or recycled back to the plasma membrane. Endocytosis is a major regulator of EGFR signaling. Therefore, elucidation of the mechanisms of EGFR endocytosis is essential for a better understanding of EGFR biology. In order to achieve a comprehensive analysis of these mechanisms, reliable methods for measuring the rates of EGFR protein turnover and the rate parameters for individual steps of EGFR endocytic trafficking must be employed. The protocols in this unit describe methodologies to measure the rates of EGFR synthesis and degradation, to monitor EGF-induced down-regulation of surface EGFR, to measure the kinetic rate parameters of internalization, recycling, and degradation of radiolabeled EGF, and to perform radioiodination of EGF by the chloramine T method.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1934-2616
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 15.14
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Quantitative analysis of endocytosis and turnover of epidermal growth factor (EGF) and EGF receptor.
pubmed:affiliation
Department of Pharmacology, University of Colorado Denver Medical School, Aurora, Colorado, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural