Linked Life Data

2023097

Source:http://linkedlifedata.com/resource/pubmed/id/2023097

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-6-4
pubmed:abstractText
The human immunodeficiency virus type 1 (HIV-1) Rev protein is a positive posttranscriptional regulator of viral structural gene expression and essential for virus replication. Rev mediates its effects through interaction with an RNA target sequence, the Rev responsive element (RRE), present within the env mRNA. Previous studies have shown that the basic stretch of amino acids are required for Rev's ability to bind RNA, whereas residues present near the carboxy terminus are essential for full biological activity. Deletion mutagenesis was used to define the minimal domain required for RNA binding and function. We found that amino acids 8 through 67 confer full binding activity, whereas full biological activity requires the presence of residues 8 through 83. The minimal RNA binding sequence of HIV-1 Rev also interacts and functions with the HIV-2 and SIV RRE elements, indicating that the same domain is responsible for the biological activity with different, but related viruses. Mutational analysis of the RRE was also carried out in an effort to further define elements crucial for its function. Our findings indicate that interaction with Rev involves a stretch of three G nucleotides present at the base of a stem loop structure previously shown to be critical for Rev binding. These results suggest that the high degree of secondary structure of the RRE RNA may serve as a guide to bring Rev in contact with a primary nucleotide sequence required for stable protein-RNA association.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0894-9255
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
558-67
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Mutational analysis of the HIV-1 Rev protein and its target sequence, the Rev responsive element.
pubmed:affiliation
Department of Molecular Oncology and Virology, Roche Research Center, Nutley, New Jersey 07110-1199.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't