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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-6-4
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pubmed:abstractText |
We have studied the consequences of introducing human recombinant transforming growth factor beta 1 (hrTGF-beta 1) into synovial tissue of the rat, to begin to better understand the significance of the fact that biologically active TGF-beta is found in human arthritic synovial effusions. Within 4-6 h after the intra-articular injection of 1 microgram of hrTGF-beta 1 into rat knee joints, extensive recruitment of polymorphonuclear leukocytes (PMNs) was observed. Cytochemistry and high resolution histological techniques were used to quantitate the influx of PMNs, which peaked 6 h post-injection. In a Boyden chamber assay, hrTGF-beta 1 at 1-10 fg/ml elicited a chemotactic response from PMNs greater in magnitude than that evoked by FMLP, establishing that TGF-beta 1 is an effective chemotactic agent for PMNs in vitro as well as in vivo. That PMNs may represent an important source of TGF-beta in inflammatory infiltrates was strongly suggested by a demonstration that stored TGF-beta 1 was secreted during phorbol myristate acetate-stimulated degranulation in vitro. Acid/ethanol extracts of human PMNs assayed by ELISA contained an average of 355 ng of TGF/beta 1 per 10(9) cells potentially available for secretion during degranulation of PMNs. [3H]Thymidine incorporation in vivo and autoradiography of tissue sections revealed that widespread cell proliferation was triggered by TGF-beta 1 injection. Synovial lining cells and cells located deep within the subsynovial connective tissue were identified as sources of at least some of the new cells that contribute to TGF-beta 1-induced hyperplasia. Our results demonstrate that TGF-beta is capable of exerting pathogenic effects on synovial tissue and that PMNs may represent a significant source of the TGF-beta present in synovial effusions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-1084749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-1688578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-1696268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2109776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2143773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2177343,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2242422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2295877,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2395327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2424019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2460474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2478145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2479033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2527268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2542408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2586525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2663990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2708352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2710133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2745570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2810254,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2871125,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2886992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2888109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2897367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2909658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2922705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-2982829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3041283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-307472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3091636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3129508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3308901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3422482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3470308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3476497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3491869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-3495542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-4351807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-4568301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-6208555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-6251462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-6429145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-6602130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-6957865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2022923-7062727
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1121-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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