Linked Life Data

2022690

Source:http://linkedlifedata.com/resource/pubmed/id/2022690

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-6-5
pubmed:abstractText
Insulin gene expression in rat insulinoma (RIN) cells is extinct in RIN x fibroblast hybrids and can reappear upon loss of DNA contributed by the fibroblast parent. (Besnard et al., Exp. Cell Res. 185:101-108, 1989). In the present study, we looked for the role of 5'-flanking sequences of the human insulin gene in the negative control observed in the hybrids. RIN cells were transformed with composite genes which consisted of the coding sequence of the gpt gene placed under the control of 5'-flanking regions of the human insulin gene (Ins.gpt gene). Upon hybridization of these cells with mouse fibroblasts, the expression of both Ins.gpt and endogenous rat insulin genes were suppressed together. The results obtained indicate that cis-acting DNA elements are involved in the negative control of the gene. These elements are located in a fragment spread from -258 to +241 of the transcription origin of the human insulin gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
349-55
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Extinction of the human insulin gene expression in insulinoma x fibroblast somatic cell hybrids involves cis-acting DNA elements.
pubmed:affiliation
Unité INSERM 257, Institut Jacques Monod du CNRS, Université Paris VII, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't