Source:http://linkedlifedata.com/resource/pubmed/id/20222070
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2010-3-16
|
| pubmed:abstractText |
The solid-phase synthesis (SPS) of a structurally complex glycopeptide, using Sieber amide resin, was monitored by high resolution magic angle spinning NMR, demonstrating the further application of this technique. A synthetic peptidoglycan derivative, a precursor of a biologically active PGN, known to be involved in the cellular recognition, was prepared by SPS. The synthesis involved the preparation of an N-alloc glucosamine moiety and the synthesis of a simple amino acid sequence L-Ala-D-Glu-L-Lys-D-Ala-D-Ala. Last step consisted the coupling, on solid-phase, of the protected muramyl unit to the peptide chain. Proton spectra with good suppression of the polystyrene signals in swollen resin samples were obtained in DMF-d(7) as a solvent and by using a nonselective 1D TOCSY/DIPSI-2 scheme, thus allowing to follow the SPS without losses of compound and cleavage from the resin. The assignment of the proton spectra of the resin-bound amino acid sequence and of the bound glycopeptide was achieved through the combination of MAS COSY, TOCSY and NOESY.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
1097-458X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2010 John Wiley & Sons, Ltd.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
48
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
323-30
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| pubmed:meshHeading | |
| pubmed:year |
2010
|
| pubmed:articleTitle |
Application of HR-MAS NMR in the solid-phase synthesis of a glycopeptide using Sieber amide resin.
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| pubmed:publicationType |
Letter,
Research Support, Non-U.S. Gov't
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