Source:http://linkedlifedata.com/resource/pubmed/id/20202418
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2010-3-5
|
| pubmed:abstractText |
Efforts to identify wildlife reservoirs for tick-borne pathogens are frequently limited by poor understanding of tick-host interactions and potentially transient infectivity of hosts under natural conditions. To identify reservoir hosts for lone star tick (Amblyomma americanum)-associated pathogens, we used a novel technology. In field-collected ticks, we used PCR to amplify a portion of the 18S rRNA gene in remnant blood meal DNA. Reverse line blot hybridization with host-specific probes was then used to subsequently detect and identify amplified DNA. Although several other taxa of wildlife hosts contribute to tick infection rates, our results confirm that the white-tailed deer (Odocoileus virginianus) is a reservoir host for several A. americanum-associated pathogens. Identification of host blood meal frequency and reservoir competence can help in determining human infection rates caused by these pathogens.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1080-6059
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
433-40
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| pubmed:meshHeading |
pubmed-meshheading:20202418-Animals,
pubmed-meshheading:20202418-Animals, Wild,
pubmed-meshheading:20202418-Blood,
pubmed-meshheading:20202418-Borrelia,
pubmed-meshheading:20202418-DNA, Bacterial,
pubmed-meshheading:20202418-DNA Probes,
pubmed-meshheading:20202418-Deer,
pubmed-meshheading:20202418-Disease Reservoirs,
pubmed-meshheading:20202418-Ehrlichia,
pubmed-meshheading:20202418-Feeding Behavior,
pubmed-meshheading:20202418-Host-Pathogen Interactions,
pubmed-meshheading:20202418-Humans,
pubmed-meshheading:20202418-Ixodidae,
pubmed-meshheading:20202418-Nucleic Acid Hybridization,
pubmed-meshheading:20202418-Polymerase Chain Reaction,
pubmed-meshheading:20202418-RNA, Ribosomal, 16S,
pubmed-meshheading:20202418-RNA, Ribosomal, 18S,
pubmed-meshheading:20202418-Sequence Analysis, DNA,
pubmed-meshheading:20202418-Species Specificity,
pubmed-meshheading:20202418-Tick Infestations
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Blood meal analysis to identify reservoir hosts for Amblyomma americanum ticks.
|
| pubmed:affiliation |
Washington University in St Louis, Missouri 63025, USA. ballan@wustl.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|