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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2010-10-27
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pubmed:abstractText |
The toxic interaction of melamine with herring sperm DNA (hs-DNA) was investigated by using fluorescence and UV-vis absorption spectra techniques. The experimental results showed that the toxic interaction between melamine and hs-DNA occurred. Fluorescence quenching experiments indicated the existence of electrostatic binding between melamine and hs-DNA. The binding constants K(A) and the binding site numbers were calculated by means of the Stern-Volmer equation and were 9.8 × 10(4) L mol(-1) and 1.3, respectively. Both the results of fluorescence spectra and UV-vis absorption spectra verified that there are electrostatic binding between melamine and hs-DNA. The possibility in the presence of a classical intercalation binding mode could be ruled out by using DNA unwinding experiments.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1099-0461
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Wiley Periodicals, Inc.
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pubmed:issnType |
Electronic
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
323-9
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pubmed:dateRevised |
2011-3-18
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pubmed:meshHeading |
pubmed-meshheading:20196162-Absorption,
pubmed-meshheading:20196162-Animals,
pubmed-meshheading:20196162-Binding Sites,
pubmed-meshheading:20196162-DNA,
pubmed-meshheading:20196162-Fishes,
pubmed-meshheading:20196162-Fluorescence,
pubmed-meshheading:20196162-Intercalating Agents,
pubmed-meshheading:20196162-Male,
pubmed-meshheading:20196162-Resins, Synthetic,
pubmed-meshheading:20196162-Spectrometry, Fluorescence,
pubmed-meshheading:20196162-Spectrophotometry, Ultraviolet,
pubmed-meshheading:20196162-Spectrum Analysis,
pubmed-meshheading:20196162-Spermatozoa,
pubmed-meshheading:20196162-Static Electricity,
pubmed-meshheading:20196162-Triazines
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pubmed:articleTitle |
Spectroscopic investigation on the toxic interaction of melamine with herring sperm DNA.
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pubmed:affiliation |
School of Environmental Science and Engineering, Shandong University, Jinan 250100, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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