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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
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|
pubmed:dateCreated |
1991-5-23
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|
pubmed:abstractText |
Isolated guinea pig myocytes were loaded with the Na(+)-sensitive fluorescent probe, sodium-binding benzofuran isophthalate (SBFI). The 340/380 nm fluorescence ratios were measured with fluorescence microscopy. The distribution of intracellular Na+ concentration ([Na+]i) was homogenous, and the mean resting [Na+]i was 8.4 +/- 0.5 mM. There was a significant relationship (r = 0.66, p less than 0.001) between elevation of [Na+]i and shortening of longitudinal length of the cells, during the perfusion of 100 microM strophanthidin. It is concluded that this method is suitable for measuring [Na+]i in isolated myocytes.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0006-291X
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
15
|
|
pubmed:volume |
175
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
611-6
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|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2018505-Animals,
pubmed-meshheading:2018505-Benzofurans,
pubmed-meshheading:2018505-Cytoplasm,
pubmed-meshheading:2018505-Ethers, Cyclic,
pubmed-meshheading:2018505-Fluorescent Dyes,
pubmed-meshheading:2018505-Guinea Pigs,
pubmed-meshheading:2018505-Microscopy, Fluorescence,
pubmed-meshheading:2018505-Muscles,
pubmed-meshheading:2018505-Sodium,
pubmed-meshheading:2018505-Spectrometry, Fluorescence,
pubmed-meshheading:2018505-Strophanthidin
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|
pubmed:year |
1991
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|
pubmed:articleTitle |
Quantification of intracellular free sodium ions by using a new fluorescent indicator, sodium-binding benzofuran isophthalate in guinea pig myocytes.
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|
pubmed:affiliation |
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro
|
