Linked Life Data

20171893

Source:http://linkedlifedata.com/resource/pubmed/id/20171893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-3-8
pubmed:abstractText
Monosialoganglioside GM1 is a known neurotrophic factor. Nerve growth factor (NGF), a member of the neurotrophin family, is important for the survival, differentiation and maturation of neurons. The aim of this study was to test whether administration of GM1 and NGF can ameliorate glutamate (Glu) neurotoxicity in primary cultured embryonic rat dorsal root ganglia (DRG) and spinal cord neurons, and to investigate the mechanism underlying any effect. DRG and spinal cord neurons were exposed to the following treatments: Glu (2 mmol/L); Glu (2 mmol/L) plus GM1 (10mg/mL); Glu (2 mmol/l) plus NGF (10 ng/mL); Glu (2 mmol/L) plus GM1 (5mg/mL) and NGF (5 ng/mL). Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, ultrastructural alterations were examined using inverse phase contrast microscopy and electron microscopy, mitochondrial membrane potential was measured using rhodamine 123 labeling and flow cytometry, and neurofilament light (NF-L) mRNA expression was detected by reverse transcription-polymerase chain reaction. It was found that GM1 and NGF can increase the viability of neurons incubated with Glu, which, after GM1 and NGF treatment, were almost morphologically normal. The mitochondrial membrane potential of neurons was lowest for neurons treated with Glu alone, and that for neurons treated with Glu plus GM1 and NGF was higher than that for treatment with GM1 or NGF alone. The mRNA of NF-L was expressed at the highest level in neurons treated with Glu plus GM1 and NGF. Our findings indicate that NGF and GM1 act synergistically to protect DRG and spinal cord neurons from Glu cytotoxicity. NGF and GM1 may function by maintaining normal mitochondrial membrane potential or by promoting NF-L mRNA expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1532-2653
pubmed:author
pubmed:copyrightInfo
(c) 2009. Published by Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
495-500
pubmed:meshHeading
pubmed-meshheading:20171893-Animals, pubmed-meshheading:20171893-Cell Separation, pubmed-meshheading:20171893-Cell Survival, pubmed-meshheading:20171893-Cells, Cultured, pubmed-meshheading:20171893-Drug Synergism, pubmed-meshheading:20171893-Flow Cytometry, pubmed-meshheading:20171893-G(M1) Ganglioside, pubmed-meshheading:20171893-Ganglia, Spinal, pubmed-meshheading:20171893-Glutamic Acid, pubmed-meshheading:20171893-Membrane Potential, Mitochondrial, pubmed-meshheading:20171893-Microscopy, Electron, Transmission, pubmed-meshheading:20171893-Nerve Growth Factor, pubmed-meshheading:20171893-Neurofilament Proteins, pubmed-meshheading:20171893-Neurons, pubmed-meshheading:20171893-Neuroprotective Agents, pubmed-meshheading:20171893-RNA, Messenger, pubmed-meshheading:20171893-Rats, pubmed-meshheading:20171893-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20171893-Spinal Cord
pubmed:year
2010
pubmed:articleTitle
GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure.
pubmed:affiliation
Department of Human Anatomy, Binzhou Medical College, 346 Guanhai Road, Laishan, Yantai 264003, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't