rdf:type |
|
lifeskim:mentions |
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pubmed:issue |
2
|
pubmed:dateCreated |
2010-3-1
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pubmed:abstractText |
Autophagy is known to be important in presentation of cytosolic antigens on MHC class II (MHC II). However, the role of autophagic process in antigen presentation in vivo is unclear. Mice with dendritic cell (DC)-conditional deletion in Atg5, a key autophagy gene, showed impaired CD4(+) T cell priming after herpes simplex virus infection and succumbed to rapid disease. The most pronounced defect of Atg5(-/-) DCs was the processing and presentation of phagocytosed antigens containing Toll-like receptor stimuli for MHC class II. In contrast, cross-presentation of peptides on MHC I was intact in the absence of Atg5. Although induction of metabolic autophagy did not enhance MHC II presentation, autophagic machinery was required for optimal phagosome-to-lysosome fusion and subsequent processing of antigen for MHC II loading. Thus, our study revealed that DCs utilize autophagic machinery to optimally process and present extracellular microbial antigens for MHC II presentation.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/AI054359,
http://linkedlifedata.com/resource/pubmed/grant/AI062428,
http://linkedlifedata.com/resource/pubmed/grant/AI064705,
http://linkedlifedata.com/resource/pubmed/grant/AI72627,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-02S1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-06A2,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-06A2S1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-07,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-07S1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-08,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI054359-09,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI062428-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI062428-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI062428-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI062428-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI062428-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI062428-06A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI062428-07,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI064705-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI064705-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI064705-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI064705-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI064705-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI064705-07,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI081884-01A2,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI081884-02,
http://linkedlifedata.com/resource/pubmed/grant/R56 AI062428-06
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1097-4180
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pubmed:author |
|
pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
26
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
227-39
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pubmed:dateRevised |
2011-10-24
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pubmed:meshHeading |
pubmed-meshheading:20171125-Animals,
pubmed-meshheading:20171125-Antigen Presentation,
pubmed-meshheading:20171125-Cells, Cultured,
pubmed-meshheading:20171125-Dendritic Cells,
pubmed-meshheading:20171125-Female,
pubmed-meshheading:20171125-Herpes Simplex,
pubmed-meshheading:20171125-Herpesvirus 2, Human,
pubmed-meshheading:20171125-Histocompatibility Antigens Class II,
pubmed-meshheading:20171125-Lymphocyte Activation,
pubmed-meshheading:20171125-Mice,
pubmed-meshheading:20171125-Mice, Inbred C57BL,
pubmed-meshheading:20171125-Mice, Knockout,
pubmed-meshheading:20171125-Microtubule-Associated Proteins,
pubmed-meshheading:20171125-RNA, Small Interfering,
pubmed-meshheading:20171125-Radiation Chimera
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pubmed:year |
2010
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pubmed:articleTitle |
In vivo requirement for Atg5 in antigen presentation by dendritic cells.
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pubmed:affiliation |
Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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