Linked Life Data

20151168

Source:http://linkedlifedata.com/resource/pubmed/id/20151168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-2-19
pubmed:abstractText
High sensitivity of IgG assays is required in order to diagnose Toxoplasma infection in immunocompromised patients and women before and during early pregnancy. Positive results obtained in new highly sensitive Toxoplasma IgG assays have been observed in sera that gave negative results in reference assays. To allow the resolution of these discrepancies, we developed a neutralization assay using 80 microg/ml of a soluble membrane fraction of Toxoplasma in the automated Elecsys IgG assay. We were able to block the detection of Toxoplasma IgG antibodies in 283 sera with IgG titers ranging from below the limit of detection to >2,300 IU/ml. The mean percentage of neutralization was 88%, with a minimum neutralization of 77%. In summary, the neutralization assay represents a valuable tool to confirm the specificity of Toxoplasma IgG antibodies in the sera of immunocompromised patients and women before and during early pregnancy. Discrepancies between positive results in highly sensitive Toxoplasma IgG assays and negative results in reference assays can, thereby, be resolved and may allow a decision to be made on the management of patients.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1435-4373
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
359-63
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Neutralization assay to resolve discrepancies between positive results in new highly sensitive anti-Toxoplasma gondii IgG assays and negative results in reference tests.
pubmed:affiliation
Roche Diagnostics GmbH, Nonnenwald 2, 82377 Penzberg, Germany.
pubmed:publicationType
Journal Article