Linked Life Data

20149852

Source:http://linkedlifedata.com/resource/pubmed/id/20149852

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-3-22
pubmed:abstractText
To meet the increasing need of simultaneously co-expressing two different genes in the same cell of transgenic Caenorhabditis elegans, here, we report the establishment of dicistronic vectors that contain an intercistronic region (ICR) of the C. elegans operon, CEOP5428. In these vectors, a green fluorescence protein (GFP) and a red FP (RFP) genes were placed in the first and second cistrons, respectively, which were separated by the ICR. Driven by the fibrillarin (fib-1) or myo-2 promoter, the GFP- and RFP-fusion proteins were consistently co-expressed in the entire worm cells or in the pharynx muscle cells of the transgenic worms, respectively. Our work demonstrates that ICR-containing dicistronic vectors could be developed into versatile co-expression systems in C. elegans for functional analysis of genes of interest.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1879-0038
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
455
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-21
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Vectors for co-expression of two genes in Caenorhabditis elegans.
pubmed:affiliation
Department of Life Science, Chang Gung University, Tao-Yuan, Taiwan. leeliwei@msu.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't