Linked Life Data

20149613

Source:http://linkedlifedata.com/resource/pubmed/id/20149613

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-4-20
pubmed:abstractText
The present study was to evaluate the effects of Ardipusilloside I isolated from Ardisia pusilla on the growth, vascular endothelial growth factor receptor (VEGFR) expression and apoptosis of NCI-H460 cell line by MTT, ELISA and flow cytometer, respectively. The docking assay between Ardipusilloside I and VEGFR was studied by Sybyl/Sketch module. The change of microstructure was observed by transmission electron microscope (TEM). DNA fragmentation was visualized by agarose gel electrophoresis. The protein expression of Bax and Bcl-2 was detected by immunohistochemistry (IHC). A series of changes were observed in NCI-H460 cell treated by Ardipusilloside I, including microstructure, DNA fragmentation, protein expression of VEGFR, Bax and Bcl-2. The results showed Ardipusilloside I had a good docking with VEGFR and could inhibit growth and induce apoptosis of NCI-H460 cell in a dose-dependent manner. Cell cycle was significantly stopped at the G(1) phase. Under electronic microscope, the morphology of NCI-H460 cell treated with Ardipusilloside I showed nuclear karyopycnosis, chromatin agglutination and typical apoptotic body. VEGFR and Bcl-2 expression were decreased and Bax expression was increased. In conclusion, all these results demonstrate that Ardipusilloside I has a good docking with VEGFR and has an inhibitory effect on growth of NCI-H460 cell and can induce its apoptosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1618-095X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
519-26
pubmed:meshHeading
pubmed-meshheading:20149613-Antineoplastic Agents, Phytogenic, pubmed-meshheading:20149613-Apoptosis, pubmed-meshheading:20149613-Ardisia, pubmed-meshheading:20149613-Binding, Competitive, pubmed-meshheading:20149613-Cell Line, pubmed-meshheading:20149613-Cell Nucleus, pubmed-meshheading:20149613-DNA Breaks, pubmed-meshheading:20149613-Humans, pubmed-meshheading:20149613-Lung Neoplasms, pubmed-meshheading:20149613-Microscopy, Electron, Transmission, pubmed-meshheading:20149613-Molecular Structure, pubmed-meshheading:20149613-Oleanolic Acid, pubmed-meshheading:20149613-Plant Extracts, pubmed-meshheading:20149613-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:20149613-Receptors, Vascular Endothelial Growth Factor, pubmed-meshheading:20149613-Saponins, pubmed-meshheading:20149613-bcl-2-Associated X Protein
pubmed:year
2010
pubmed:articleTitle
Ardipusilloside I purified from Ardisia pusilla competitively binds VEGFR and induces apoptosis in NCI-H460 cells.
pubmed:affiliation
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't