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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-3-10
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pubmed:abstractText |
The present study performed proteomic analysis of the midbrain and striatum of 6-hydroxydopamine (6-OHDA)-treated neonatal rats--a model of attention-deficit hyperactivity disorder (ADHD). Proteomic analysis revealed that a 58-kDa glucose-regulated protein (Grp58) was temporarily phosphorylated and its level was elevated by 6-OHDA. Furthermore, 6-OHDA increased the expression level of C/EBP homologous protein (CHOP), a mediator of endoplasmic reticulum (ER) stress response, in the midbrain and striatum. In vitro experiments using PC12 cells revealed that 6-OHDA or hydrogen peroxide could induce the elevation of Grp58 and CHOP. 6-OHDA could induce the elevation of Grp58 and CHOP in the presence of catalase, a hydrogen peroxide-removing enzyme, suggesting that the elevation of Grp58 and CHOP are induced by both hydrogen peroxide and p-quinone generated by 6-OHDA. Collectively, these findings suggest that ER stress involving the alteration of Grp58 and CHOP play a significant role in the induction of insults by 6-OHDA in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Catalase,
http://linkedlifedata.com/resource/pubmed/chemical/Ddit3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Grp58 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidopamine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Disulfide-Isomerases,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor CHOP,
http://linkedlifedata.com/resource/pubmed/chemical/benzoquinone
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1029-2470
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
410-21
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pubmed:meshHeading |
pubmed-meshheading:20109102-Animals,
pubmed-meshheading:20109102-Animals, Newborn,
pubmed-meshheading:20109102-Basal Ganglia,
pubmed-meshheading:20109102-Benzoquinones,
pubmed-meshheading:20109102-Caspases,
pubmed-meshheading:20109102-Catalase,
pubmed-meshheading:20109102-Chromatography, Liquid,
pubmed-meshheading:20109102-Dopamine,
pubmed-meshheading:20109102-Dose-Response Relationship, Drug,
pubmed-meshheading:20109102-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:20109102-Endoplasmic Reticulum,
pubmed-meshheading:20109102-Hydrogen Peroxide,
pubmed-meshheading:20109102-Male,
pubmed-meshheading:20109102-Mesencephalon,
pubmed-meshheading:20109102-Neurons,
pubmed-meshheading:20109102-Oxidopamine,
pubmed-meshheading:20109102-PC12 Cells,
pubmed-meshheading:20109102-Phosphorylation,
pubmed-meshheading:20109102-Protein Disulfide-Isomerases,
pubmed-meshheading:20109102-Proteomics,
pubmed-meshheading:20109102-Rats,
pubmed-meshheading:20109102-Rats, Wistar,
pubmed-meshheading:20109102-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:20109102-Tandem Mass Spectrometry,
pubmed-meshheading:20109102-Time Factors,
pubmed-meshheading:20109102-Transcription Factor CHOP,
pubmed-meshheading:20109102-Up-Regulation
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pubmed:year |
2010
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pubmed:articleTitle |
Proteomic characterization of the striatum and midbrain treated with 6-hydroxydopamine: alteration of 58-kDa glucose-regulated protein and C/EBP homologous protein.
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pubmed:affiliation |
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Kansai Center, Ikeda, Osaka, Japan.
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pubmed:publicationType |
Journal Article
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