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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-2-17
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pubmed:abstractText |
Photoactivatable penetratin analogues bearing three different photoprobes, which do not disturb the membranotropic properties of the peptides, have been tested for their photo-cross-linking efficacy in glycerol and lipid media. In the case of glycerol, photo-cross-linking was observed, whereas in the case of SDS (used as a membrane model system), the dynamics of the SDS/penetratin assemblies and the photosensitizer properties of the probes prevented the cross-linking between the peptide and SDS. Bilayers of DMPG were partially photo-cross-linked by the penetratin analogues containing either a benzophenone or a trifluoromethylaryl-diazirine, whereas dithienyl ketone acted exclusively as a photosensitizer. The characterization by MALDI-TOF mass spectrometry of the photoadducts formed after irradiation required basic hydrolysis of DMPG for an efficient capture of the biotinylated peptide analogues with streptavidin-coated magnetic beads. MALDI-TOF analysis of the photoadducts between the photoactivatable penetratin and DMPG allowed an unambiguous identification of the covalent bond formed with the lipids. Altogether, we show herein that the efficacy of the lipid photo-cross-linking depends on the environment, the dynamics of the supramolecular assembly, and the physicochemical properties of the photoprobe.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzophenones,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Diazomethane,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol,
http://linkedlifedata.com/resource/pubmed/chemical/Ketones,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylglycerols,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Dodecyl Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Thiophenes,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/benzophenone,
http://linkedlifedata.com/resource/pubmed/chemical/dimyristoylphosphatidylglycerol,
http://linkedlifedata.com/resource/pubmed/chemical/penetratin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1520-4812
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
17
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
352-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:20088542-Amino Acid Sequence,
pubmed-meshheading:20088542-Benzophenones,
pubmed-meshheading:20088542-Carrier Proteins,
pubmed-meshheading:20088542-Cross-Linking Reagents,
pubmed-meshheading:20088542-Diazomethane,
pubmed-meshheading:20088542-Glycerol,
pubmed-meshheading:20088542-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:20088542-Ketones,
pubmed-meshheading:20088542-Lipid Bilayers,
pubmed-meshheading:20088542-Mass Spectrometry,
pubmed-meshheading:20088542-Phosphatidylglycerols,
pubmed-meshheading:20088542-Phospholipids,
pubmed-meshheading:20088542-Photochemical Processes,
pubmed-meshheading:20088542-Sodium Dodecyl Sulfate,
pubmed-meshheading:20088542-Thiophenes,
pubmed-meshheading:20088542-Water
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pubmed:year |
2010
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pubmed:articleTitle |
Comparing lipid photo-cross-linking efficacy of penetratin analogues bearing three different photoprobes: dithienyl ketone, benzophenone, and trifluoromethylaryldiazirine.
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pubmed:affiliation |
UPMC Paris 06 - ENS - CNRS, UMR 7203, Laboratoire des BioMolecules, FR 2769 Chimie Moleculaire, Universite Pierre et Marie Curie, Case courier 182, 4, Place Jussieu, 75252 Paris Cedex 05, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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