Source:http://linkedlifedata.com/resource/pubmed/id/20086085
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-3-17
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| pubmed:abstractText |
A human Campylobacter jejuni infection model provided controlled exposure to assess vaccine efficacy and investigate protective immunity for this important diarrheal pathogen. A well-characterized outbreak strain, C. jejuni 81-176, was investigated using a volunteer experimental infection model to evaluate the dose range and duration of protection. Healthy Campylobacter-seronegative adults received C. jejuni strain 81-176 via oral inoculation of 10(5), 10(7), or 10(9) CFU (5 adults/dose), which was followed by clinical and immunological monitoring. Based on dose range clinical outcomes, the 10(9)-CFU dose (n = 31) was used to assess homologous protection at 28 to 49 days (short-term veterans [STV]; n = 8) or 1 year (long-term veterans [LTV]; n = 7) after primary infection. An illness dose effect was observed for naïve subjects (with lower doses, 40 to 60% of the subjects were ill; with the 10(9)-CFU dose, 92% of the subjects were ill) along with complete protection for the STV group and attenuated illness for the LTV group (57%). Partial resistance to colonization was seen in STV (25% of the subjects were not infected; 3-log-lower maximum excretion level). Systemic and mucosal immune responses were robust in naïve subjects irrespective of the dose or the severity of illness. In contrast, in STV there was a lack of circulating antibody-secreting cells (ASC), reflecting the local mucosal effector responses. LTV exhibited comparable ASC responses to primary infection, and anamnestic fecal IgA responses likely contributed to self-resolving illness prior to antibiotic treatment. Campylobacter antigen-dependent production of gamma interferon by peripheral blood mononuclear cells was strongly associated with protection from illness, supporting the hypothesis that TH1 polarization has a primary role in acquired immunity to C. jejuni. This study revealed a C. jejuni dose-related increase in campylobacteriosis rates, evidence of complete short-term protection that waned with time, and immune response patterns associated with protection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1098-5522
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| pubmed:author |
pubmed-author:ApplebeeLisaL,
pubmed-author:BaqarShahidaS,
pubmed-author:BourgeoisA LouisAL,
pubmed-author:BurgEdward FEF3rd,
pubmed-author:ClementsJohn DJD,
pubmed-author:GibbsPaulP,
pubmed-author:JaretR SRS,
pubmed-author:MoranAnthony PAP,
pubmed-author:OplingerMichael LML,
pubmed-author:RollinsDavidD,
pubmed-author:ScottDaniel ADA,
pubmed-author:TribbleDavid RDR,
pubmed-author:WalkerRichard IRI,
pubmed-author:WalzStevenS
|
| pubmed:issnType |
Electronic
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| pubmed:volume |
78
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1750-9
|
| pubmed:dateRevised |
2010-10-4
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| pubmed:meshHeading |
pubmed-meshheading:20086085-Administration, Oral,
pubmed-meshheading:20086085-Adult,
pubmed-meshheading:20086085-Antibodies, Bacterial,
pubmed-meshheading:20086085-Bacterial Vaccines,
pubmed-meshheading:20086085-Campylobacter Infections,
pubmed-meshheading:20086085-Campylobacter jejuni,
pubmed-meshheading:20086085-Diarrhea,
pubmed-meshheading:20086085-Feces,
pubmed-meshheading:20086085-Female,
pubmed-meshheading:20086085-Human Experimentation,
pubmed-meshheading:20086085-Humans,
pubmed-meshheading:20086085-Immunity, Mucosal,
pubmed-meshheading:20086085-Immunoglobulin A,
pubmed-meshheading:20086085-Immunologic Memory,
pubmed-meshheading:20086085-Interferon-gamma,
pubmed-meshheading:20086085-Leukocytes, Mononuclear,
pubmed-meshheading:20086085-Male,
pubmed-meshheading:20086085-Severity of Illness Index,
pubmed-meshheading:20086085-Time Factors,
pubmed-meshheading:20086085-Young Adult
|
| pubmed:year |
2010
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| pubmed:articleTitle |
Assessment of the duration of protection in Campylobacter jejuni experimental infection in humans.
|
| pubmed:affiliation |
Naval Medical Research Center, Silver Spring, Maryland, USA. dtribble@usuhs.mil
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|