Linked Life Data

20074928

Source:http://linkedlifedata.com/resource/pubmed/id/20074928

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-2-17
pubmed:abstractText
A new approach was established to construct a sandwich-type electrochemiluminescence (ECL) immunosensor by using Ru(bpy)(3)(2+)-doped silica (abbreviated as Ru-SiO(2)) nanoparticles to label secondary antibody. Firstly, carboxylate-terminated multi-walled carbon nanotubes (MWCNTs) were modified on the electrode to bond with avidin. Subsequently, biotinylated antibodies were immobilized on the surface of the electrode by employing the specific interaction of biotin/avidin and the non-covalent and covalent conjugation function of MWCNTs. Later, the electrode was incubated with antigen of mouse IgG and then reacted with the secondary antibody which was labeled by Ru-SiO(2). Accordingly, through the ECL response of Ru-SiO(2) and tripropylamine (TPA), a strong ECL signal was obtained and an amplification analysis of protein interaction was achieved. The present immunosensor showed a wide linear range of 0.05-200.00 ng mL(-1) for detecting mouse IgG, with a low detection limit of 17 pg mL(-1). There was a 4-300-fold improvement in detection limit compared with other similar studies. The morphologies of Ru-SiO(2) nanoparticles were characterized by using transmission electronic microscopy (TEM). The fabrication process of the immunosensor was studied by cyclic voltammetry (CV) and the performance of the immunosensor was monitored with an electrochemiluminescence analyzer. This new strategy for preparation of the ECL immunosensor could be easily realized and has potential application in ultrasensitive bioassays.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1873-4235
pubmed:author
pubmed:copyrightInfo
(c) 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1851-5
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Ru(bpy)3(2+)-doped silica nanoparticles labeling for a sandwich-type electrochemiluminescence immunosensor.
pubmed:affiliation
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies