Linked Life Data

20066658

Source:http://linkedlifedata.com/resource/pubmed/id/20066658

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-1-12
pubmed:abstractText
G protein-coupled receptors (GPCRs) and their signal transductions are important for both physiological and pathological processes in neuron systems. Neuronal GPCRs activated by synthetic ligands have been created by designed mutagenesis for studying their functions and signal pathways. However, these engineered GPCRs have problems, such as their high constitutive activity. To overcome this drawback, a new generation of receptors termed designer receptors exclusively activated by designer drugs (DREADDs), have been designed. DREADDs are exclusively activated by synthetic ligands, but are insensitive to their endogenous ligand and have no constitutive activity, which provides the ability to selectively modulate signal transduction of certain GPCRs in vitro and in vivo. This protocol provides detailed instructions for creating DREADDs using directed molecular evolution. The procedures to generate DREADDS include GPCR functional expression in yeast, mutant GPCR library generation, and high-throughput yeast screening. These methods are general and suitable for any GPCRs that can be functionally expressed in yeast.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1934-8576
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 4.33
pubmed:dateRevised
2011-2-14
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Generation of designer receptors exclusively activated by designer drugs (DREADDs) using directed molecular evolution.
pubmed:affiliation
University of North Carolina, Chapel Hill, North Carolina, USA.
pubmed:publicationType
Journal Article