Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-3-8
pubmed:abstractText
In this study, we obtained evidence indicating that annexin 1 is a new target of the p38/MAPKAP kinase-2 pathway and that it regulates endothelial cell migration in response to vascular endothelial growth factor (VEGF). These conclusions are supported by a series of substantiating experiments. First, by two-dimensional gel electrophoresis and mass spectrometry, we identified annexin 1 as a protein whose phosphorylation is induced by VEGF and is impaired by inhibiting p38. Second, using in vitro kinase assays and in vivo phosphorylation assays, we found that VEGF-mediated activation of LIM kinase 1 downstream of the p38 pathway triggers the phosphorylation of annexin 1. Third, VEGF-induced cell migration and tube formation in Matrigel are inhibited following small interfering RNA-mediated knockdown of annexin 1. Fourth, both processes are rescued in cells expressing an annexin 1 construct insensitive to the small interfering RNA knockdown. Finally, the VEGF/annexin 1-mediated cell migration is impaired by inhibiting p38. We therefore conclude that phosphorylation of annexin 1 regulates the angiogenic effect that is associated with the activation of the p38/LIM kinase 1 axis by VEGF.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-10608817, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-10677219, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-10744763, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-11369257, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12071847, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12090472, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12123613, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12133977, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12516535, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12639897, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12684437, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12748951, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-12777619, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-1375931, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-14516791, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-14724572, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-15133855, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-15157501, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-15168732, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-15260827, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-15821756, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16381000, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16421520, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16456544, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16574378, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16633338, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16675446, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16760434, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-16982054, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-17215481, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-17294230, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-17395884, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-17553709, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-18202108, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-18332102, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-18440775, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-19104500, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-19188496, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-19637235, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-2136806, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-7535770, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-7596435, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-7596436, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-7851416, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-8184489, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-8280084, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-8910300, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-9048659, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-9109485, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-9393975, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-9529250, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-9570758, http://linkedlifedata.com/resource/pubmed/commentcorrection/20061392-9628874
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Annexin A1, http://linkedlifedata.com/resource/pubmed/chemical/Collagen, http://linkedlifedata.com/resource/pubmed/chemical/Drug Combinations, http://linkedlifedata.com/resource/pubmed/chemical/LIMK1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Laminin, http://linkedlifedata.com/resource/pubmed/chemical/Lim Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering, http://linkedlifedata.com/resource/pubmed/chemical/VEGFA protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A, http://linkedlifedata.com/resource/pubmed/chemical/matrigel, http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1083-351X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8013-21
pubmed:dateRevised
2011-7-26
pubmed:meshHeading
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