Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-1-6
pubmed:abstractText
Early detection of cancer-associated genomic instability is crucial, particularly in tumour types in which this instability represents the essential underlying mechanism of tumourigenesis. Currently used methods require the presence of already established neoplastic cells because they only detect clonal mutations. In principle, parallel sequencing of single DNA filaments could reveal the early phases of tumour initiation by detecting low-frequency mutations, provided an adequate depth of coverage and an effective control of the experimental error. We applied ultradeep sequencing to estimate the genomic instability of individuals with hereditary non-polyposis colorectal cancer (HNPCC). To overcome the experimental error, we used an ultraconserved region (UCR) of the human genome as an internal control. By comparing the mutability outside and inside the UCR, we observed a tendency of the ultraconserved element to accumulate significantly fewer mutations than the flanking segments in both neoplastic and nonneoplastic HNPCC samples. No difference between the two regions was detectable in cells from healthy donors, indicating that all three HNPCC samples have mutation rates higher than the healthy genome. This is the first, to our knowledge, direct evidence of an intrinsic genomic instability of individuals with heterozygous mutations in mismatch repair genes, and constitutes the proof of principle for the development of a more sensitive molecular assay of genomic instability.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-10793088, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-11376800, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-11524701, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-12386821, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-12621137, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-12874782, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-15060014, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-15131266, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-15510158, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-15563510, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-15782221, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-16056220, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-16380714, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-16432518, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-16525637, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-16799556, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-16982745, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-16998490, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17043646, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17062673, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17086198, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17327285, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17344846, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17415710, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17600086, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17659080, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17702936, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17785203, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17803355, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17932254, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-17962726, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18006790, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18174240, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18176564, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18455124, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18723673, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18772396, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18772397, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-18846087, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-19252504, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-19471316, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-2039987, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-7632227, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-7894494, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8065949, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8128251, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8137274, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8261515, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8261516, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8613431, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8690195, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8764130, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-8836181, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-9111864, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-9267808, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-9385369, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-9758761, http://linkedlifedata.com/resource/pubmed/commentcorrection/20052272-9823339
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1545-7885
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e1000275
pubmed:dateRevised
2010-9-28
pubmed:meshHeading
More...