Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-12-31
pubmed:abstractText
Levamisole has previously been identified as an inhibitor of angiogenesis in vitro and in vivo, but the mechanism behind the anti-angiogenic behavior has not yet been established. However, one known effect of levamisole is the inhibition of alkaline phosphatase, and this fact encouraged us to test other phosphatase inhibitors for their anti-angiogenic effects by using the same method as used to identify levamisole: an ELISA-based co-culture angiogenesis assay giving quantitative and qualitative results. Historically, intracellular phosphatases have been associated with the downregulation of signaling pathways, and kinases with their upregulation, but lately, the phospatases have also been coupled to positive signaling, which is why inhibition of phosphatases has become associated with anti-tumorigenic and anti-angiogenic effects. The results obtained in this work reveal several agents with anti-angiogenic potential and give a strong indication that phosphatase inhibition is linked to anti-angiogenic activity. An apparent disruption of endothelial tube formation was seen for seven of eight phosphatase inhibitors tested in the angiogenesis assay. By looking at the morphological results, it was seen that most of the inhibitors impaired proliferation and elongation of the endothelial cells, which still had a differentiated appearance. One inhibitor, PTP inhibitor IV, seemed to impair endothelial cell differentiation and induced the same morphology as when cells were treated with levamisole, although at a 200 times lower concentration than that of levamisole. Hence, our work points out compounds with a potential that may be of use in the search for new medical products for the treatment of malignant tumors, or other conditions where angiogenesis plays a central role.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-10421764, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-11001068, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-11139434, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-11593409, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-11940508, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-12135675, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-12135741, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-12438248, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-12659770, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-12778132, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-12778165, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-12969022, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-14665621, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-15136787, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-15258570, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-15282661, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-15705855, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-15866871, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-16132615, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-16406676, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-16409133, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-16531064, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-16643188, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-16717135, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-1688381, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-17158450, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-17178724, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-17227708, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-17962719, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-19017914, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-2283894, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-6169, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-7896817, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-8756718, http://linkedlifedata.com/resource/pubmed/commentcorrection/20041871-9694867
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1600-0463
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-59
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Phosphatase inhibitors with anti-angiogenic effect in vitro.
pubmed:affiliation
Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark.
pubmed:publicationType
Journal Article