Source:http://linkedlifedata.com/resource/pubmed/id/20036079
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-4
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| pubmed:dateCreated |
2010-5-24
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| pubmed:abstractText |
Four sampling techniques for Mycoplasma hyopneumoniae detection, namely nasal swabbing, oral-pharyngeal brushing, tracheo-bronchial swabbing and tracheo-bronchial washing, were compared in naturally infected live pigs. In addition, a quantitative real-time PCR assay for M. hyopneumoniae quantification was validated with the same samples. 60 finishing pigs were randomly selected from a batch of contemporary pigs on a farm chronically affected by respiratory disorders. Each pig was submitted to nasal swabbing, oral-pharyngeal brushing, tracheo-bronchial swabbing and tracheo-bronchial washing. Nested-PCR and real-time PCR assays were performed on all samples. A Bayesian approach was used to analyze the nested-PCR results of the four sampling methods (i.e. positive or negative) to estimate the sensitivity and specificity of each method. M. hyopneumoniae was detected by nested-PCR in at least one sample from 70% of the pigs. The most sensitive sampling methods for detecting M. hyopneumoniae in live naturally infected pigs were tracheo-bronchial swabbing and tracheo-bronchial washing, as compared to oral-pharyngeal brushing and nasal swabbing. Swabbing the nasal cavities appeared to be the least sensitive method. Significantly higher amounts of M. hyopneumoniae DNA were found at the sites of tracheo-bronchial sampling than in the nasal cavities or at the oral-pharyngeal site (p<0.001). There was no difference between the tracheo-bronchial washing and the tracheo-bronchial swabbing results (p>0.05). Our study indicated that tracheo-bronchial swabbing associated with real-time PCR could be an accurate diagnostic tool for assessing infection dynamics in pig herds.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1873-2542
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| pubmed:author | |
| pubmed:copyrightInfo |
(c) 2009 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
14
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| pubmed:volume |
143
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
238-45
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| pubmed:meshHeading |
pubmed-meshheading:20036079-Animals,
pubmed-meshheading:20036079-Bacterial Vaccines,
pubmed-meshheading:20036079-Bacteriological Techniques,
pubmed-meshheading:20036079-Bayes Theorem,
pubmed-meshheading:20036079-Mycoplasma hyopneumoniae,
pubmed-meshheading:20036079-Pneumonia of Swine, Mycoplasmal,
pubmed-meshheading:20036079-Polymerase Chain Reaction,
pubmed-meshheading:20036079-Sensitivity and Specificity,
pubmed-meshheading:20036079-Swine
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| pubmed:year |
2010
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| pubmed:articleTitle |
Estimation of the sensitivity of four sampling methods for Mycoplasma hyopneumoniae detection in live pigs using a Bayesian approach.
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| pubmed:affiliation |
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité d'Epidémiologie et de Bien-Etre du Porc, B.P. 53, 22440 Ploufragan, France. c.fablet@ploufragan.afssa.fr
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| pubmed:publicationType |
Journal Article
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