Linked Life Data

20022606

Source:http://linkedlifedata.com/resource/pubmed/id/20022606

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2010-3-29
pubmed:abstractText
The simultaneous ultrasonic and microwave assisted extraction (UMAE) technique was first employed to obtain phenolics. The effects of UMAE variables including extraction time, microwave power, and solvent to solid radio on the yield of phenolics were investigated. The optimized conditions were as follows: solvent to solid ratio was 20:1 (ml/g), extraction time was 30s, microwave power was 500W and two times of extraction. Moreover, the phenolic yield of UMAE was higher than that by maceration, indicating a significant reduction of extraction time and an improvement of efficiency. The phenomenon is related to the strong disruption of leaf tissue structure by microwave induced expansion and ultrasonic shaking, which had been observed with the scanning electron microscopy. The phenolic compositions of the extract was then identified by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), 10 compounds had been characterized, providing a more complete identification of phenolic compounds in burdock leaves than previously reported. The occurrence of benzoic acid and p-coumaric acid is reported for the first time. This study suggests that UMAE is a good alternative for the extraction of phenolics, with a great potential for industrial application. Also, UMAE provides a new sample preparation technique for characterization of the phenolic compounds from plants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1873-3778
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
16
pubmed:volume
1217
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2441-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Improved extraction and identification by ultra performance liquid chromatography tandem mass spectrometry of phenolic compounds in burdock leaves.
pubmed:affiliation
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't